Manufacturing Method & Technology

Application of Freeze-drying manufacturing method in cosmetics

Freeze Drying

The stability of human stem cell culture solutions and growth factors deteriorates over time, which causes the gradual loss of efficacy. We have applied a special treatment using freeze-drying technology on beauty lotions, serums, and emulsions with beauty components compounded, which are then thawed again to make the product (two-component cosmetics for preparation before use). This manufacturing method is characterized by the retention of the original form, minimizing changes in color and taste. In addition, there are many advantages such as the residual fine gaps in the areas of crystallized ice which allows the added water to permeate, resulting in high recovery and reproducibility when returned to liquid form. However, the application of this technology in cosmetics was delayed because of the difficulty in clarifying the temperature range and period for efficient freezing. We have utilized our expert technology and experience in enabling freeze-drying of cosmetics.

Absolutely no preservatives added

No Preservative

Our unique freeze-drying technology allows not only the non-addition of preservatives but the absolute non-addition of preservatives. Since these products come directly in contact with your delicate skin, experienced personnel conduct many of the processes manually. The manufacturing process is carried out carefully with hygiene measures enforced for the air conditioning on the premises and the devices used for manufacture.

Nano-liposome encapsulation technology

Encapsulation Technology

Nano-liposome , which is a type of encapsulation technology allows the stabilization of unstable components such as EGF (epidermal cell growth factor) and FGF (fibroblast growth factor). The permeability is higher than liposomes, which makes it easier to blend with the skin and remain in the keratin layer, as well as supplying the skin with active ingredients over a long period of time.

Main advantages of encapsulation

  • Merit 1: Improved permeation efficiency

    Strong permeation capacity through the epidermis (keratin layer) allows delivery of the active ingredients underneath the epidermis.

  • Merit 2: Decreased skin stimulation

    Encapsulation of active ingredients with skin irritability (such as retinol) allows the stimulation to be reduced dramatically.

  • Merit 3: Improved active ingredient stability

    Prevents the degradation of active ingredients on the skin and in the body and retains optimal concentration over prolonged period, allowing the effect to be sustained for a long period of time.

Differences between a liposome and a nano-liposome

  Liposome(existing technology) Nano-liposome(new permeation technology)
Ingredient Phospholipids DAG-PEG Lipid
Permeation efficiency 10 10
Encapsulation efficiency ~55% ~90%
Productivity Low High
Stability Low High

Liposome is a substance with size of about 100 to 130 nm and has multi-molecular structure like the cell membranes found in our bodies. The multilayer structure allows the water-soluble substances to be trapped in the water layer and oil-soluble substances to be trapped in the oil layer. Liposome is useful in nano-size, and the drug can be encapsulated in a small capsule and allowed to be absorbed and permeated into the body. The cell membrane in the body is in a barrier form that prevents the permeation of water, therefore proteins and genes cannot cross the membrane. However, since the structure of liposome is similar to that of the cell membrane, it fuses with the cell membrane after entering the body, which then allows the active ingredients dissolved in the water inside to be absorbed by the cells. The concept of DDS involves the stabilization of drugs by absorption of active ingredients in liposome, which is then able to cross the barrier zone. Liposomes are produced from egg yolks or soybean lecithin and are utilized in areas such as gene therapy.

Three challenges that made liposome capsules unsuitable for cosmetics

Difficulty in compounding in products while maintaining the capsule structure
Difficulty in enveloping water-soluble active ingredients in the capsules
Existing liposome components cause degeneration of amino acids.

In order to overcome these three challenges, we use low-molecular collagen as the constituent and adopted the micro-fluid manufacturing method to create the nano-liposome capsules. Various growth factors and active substances such as SOD found in stem cell culture fluid are in the form of polypeptides, which involve binding of the tens to hundreds of amino acids in a similar manner to thread tangles. These are aggregated to protein in yarn-ball form (high molecular collagen), which is then surrounded by low molecular collage and ceramide polymers by the micro-fluid manufacturing method to create the liposome structure. As a result, the issues of fragility and degeneration of amino acid components were resolved, and liposome capsules were found to retain the effect for 2 and 3 years within the cosmetic product.